Proteins nqa tawm cov nyom biochemical thiab yuav tsum muaj ib qho tshwj xeeb-seem-seem spatial qauv thaum ua haujlwm microbial. Tom qab microbial tiam, protein lawv tus kheej kuj tau yauv ua cov txheej txheem ua physiological tshuab hluav taws xob. Hauv cov kev sim biochemical, feem ntau nws yog feem ntau tsim nyog los ua kev tshawb fawb science ntawm cov protein denaturation. Guanidine hydrochloride thiab urea yog feem ntau siv kev sim ntsuas kev sim hauv protein denaturation. Yog li, dab tsi yog qhov sib txawv ntawm ob? Yuav ua li cas xaiv hauv kev sim?
Vim tias kev ua tau zoo ntawm lwm yam kev hloov pauv ntawm cov khoom siv ntshiab uas muaj txiaj ntsig thiab cov kev hloov pauv ntawm lawv lub cev thiab tshuaj lom neeg. Cov xwm txheej no yog hu ua cov protein denaturation. Kev hloov pauv tuaj yeem koom nrog qhov sib xyaw ua ke ntawm theem nrab thiab disulfide bonds, tab sis tsis yog qhov sib xyaw peptide ntawm cov qauv txheej txheem.
Guanidine hydrochloride thiab urea Transagerender proteins muaj ob lub tshuab: thawj qhov system yog qhov uas nyiam kev hloov pauv thiab urea, tsim cov khoom siv offhales. Thaum nitric oxide synthease raug tshem tawm, nws qhia txog kev ua haujlwm nyob rau hauv kev sib npaug, nrog kev nce ntxiv hauv kev nce ntawm tus neeg sawv cev denaturing. Cov protein nyob rau hauv cov huab cua ntshiab txuas ntxiv mus hloov mus rau hauv nitric oxide synthease, kawg ua kom tiav protein transiger; Qhov thib ob hauv cov txiaj ntsig zoo ntawm guanidine hydrochloride thiab urea ntawm hydrophobic amino acid seem. Vim tias muaj peev xwm ntawm guanidine hydrochloride thiab urea los ua kev sib tsoo hydrocidoride thiab urea yuav tsum tau tawg hauv siab ({} mol \/ l) kev daws teeb meem. Raws li qhov tshwm sim, guanidine hydrochloride thiab urea ua cov qauv organic rau hauv cov protein molecular seem, ua rau cov protein ntau, ua rau cov protein sib txawv.
Qhov sib txawv ntawm ob hauv protein hloov dua siab tshiab:
Cov neeg muaj nuj nqis siab: ntawm sab hauv tsev kub, 3-2 mol \/ u guanidine wydrochloride tuaj yeem ua rau lawv lub ntuj ntuj rau hauv nruab nrab ntawm lawv lub xeev hloov pauv. Feem ntau, nce cov txiaj ntsig zoo ntawm cov neeg sawv cev denaturing tuaj yeem txhim kho qib kev hloov pauv tuaj yeem hloov protein kom tiav rau nws lub xeev hloov pauv. Guanidine hydrochloride muaj cov peev xwm denaturation tsis muaj zog dua li urea vim nws lub zog cationic. Qee cov tshuaj pleev ib ce speins tsis tuaj yeem hloov pauv txawm tias hauv 8 mol \/ l urformed uas lawv feem ntau muaj nyob rau hauv 8 mol \/ u guanidine delochloride tov.
Solubility: lub solubility ntawm urea yog qeeb thiab tsis muaj zog tshaj li ntawd ntawm hydrocidine hydrochloride, nrog rau solubility ntawm 70% ~ 90%. Thaum urea muaj lub sijhawm ua tau zoo dua, nws tawg kom tsim cov cuab yeejy, uas tau kho kom zoo nkauj rau cov pab pawg hydboxyl ntawm cov khoom siv roj Asset Txawm li cas los xij, nws muaj qhov zoo ntawm tsis yog tsis muaj zog hauv electrolytes, nruab nrab, thiab tus nqi tsawg; Guanidine hydrochloride muaj cov solubility muaj ntau dua 95% thiab cov txiaj ntsig ceev ceev yam tsis muaj kev sib haum xeeb ntawm cov khoom muaj protein ntau. Txawm li cas los xij, nws muaj qhov tsis zoo xws li tus nqi siab dua piv rau urea, kev daws teeb meem nyob rau hauv acidic thiab alkaline cov qauv, thiab muaj peev xwm cuam tshuam rau kev sim hoc kev sim.
Zuag qhia tag nrho, raws li cov txheej txheem uas muaj txiaj ntsig zoo hauv cov protein thiab uroan nyuaj thiab uranidine hydrochloride muaj cov kev sib cav sib ceg Txawm li cas los xij, nws muaj cov kev teeb meem xws li nce tus nqi, yooj yim nyob rau hauv cov acid-paus qauv, thiab cuam tshuam rau cov protein ion txauv cov roj ntsha; Urea muaj kev tsis zoo solubility, tab sis nws muaj qhov zoo xws li tsis yog qhov tsis muaj zog electrolyte, thiab tsis yooj yim ua rau muaj ntau cov protein los khom tom qab protein refolding. Hauv kev sim tshwj xeeb, cov kws tshawb fawb txuj ci yuav tsum xaiv raws cov qauv thiab cov hom phiaj kom tau txais cov txiaj ntsig kev sim zoo tshaj plaws.